{"id":41720,"date":"2022-01-06T13:25:00","date_gmt":"2022-01-06T13:25:00","guid":{"rendered":"https:\/\/www.alicat.com\/?p=41720"},"modified":"2026-01-14T18:45:50","modified_gmt":"2026-01-14T18:45:50","slug":"schuttelwippe-ruhrkessel-bioreaktoren","status":"publish","type":"post","link":"https:\/\/www.alicat.com\/de\/articles\/shaker-rocker-stirred-tank-bioreactors\/","title":{"rendered":"Sch\u00fcttel-, Wipp- und R\u00fchrkessel-Bioreaktoren - die Vorteile der einzelnen Verfahren"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.24.2″ _module_preset=”default” custom_css_free_form=”@media (max-width: 375px) {|| .et-db #et-boc .et-l .et_pb_post_content_0_tb_body {|| width:100% !important;|| }}” locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”2em||0px||false|false” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_heading title=”Shakers, Rockers, and Stirred Tank Bioreactors \u2013 Advantages of Each” _builder_version=”4.27.4″ _module_preset=”default” custom_css_free_form=”selector h1 {|| text-wrap: pretty;||}” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_divider color=”#2A3B47″ divider_weight=”2.5px” _builder_version=”4.27.4″ _module_preset=”default” height=”0px” custom_margin=”3rem||3rem||true|false” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”0px||0px||true|false” locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]We\u2019ve previously written articles comparing single\u2011use bioreactors to stainless steel or glass multi\u2011use bioreactors. You can read about when to switch to single\u2011use<\/a>, the advantages of single\u2011use in small\u2011scale bioprocessing<\/a>, and the environmental impacts<\/a> of single- and multi\u2011use bioreactors. In this article, we compare the following mixing technologies: shaker, rocker, and stirred tank bioreactors. At Interphex 2021 we had the opportunity to speak with engineers from Kuhner Shaker<\/a> about their new shaker bioreactors, MicroDigital Co.<\/a> about their single\u2011use Cel Bic rocker bioreactors, and Eppendorf about their stirred tank bioreactors. Each of these bioreactor technologies can be used with single\u2011use or reusable bioreactors, differing primarily in their method of aerating and mixing the culture to maximize oxygen transfer from the bioreactor environment into the growing cells.[\/et_pb_text][et_pb_heading title=”Stirred tank bioreactors” _builder_version=”4.27.4″ _module_preset=”default” title_level=”h3″ global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_image src=”https:\/\/www.alicat.com\/wp-content\/uploads\/2024\/05\/Types-of-Bio-Mixing-Stirring.webp” alt=”Stirring bioreactor representation” _builder_version=”4.27.4″ _module_preset=”default” max_width=”25%” max_width_tablet=”25%” max_width_phone=”50%” max_width_last_edited=”on|phone” module_alignment=”center” custom_margin=”8px||||false|false” custom_padding=”|2rem|2rem||false|false” custom_padding_tablet=”|2rem|2rem||false|false” custom_padding_phone=”|0rem|||false|false” custom_padding_last_edited=”on|phone” custom_css_main_element=”float: left;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”float: none;” custom_css_main_element_tablet=”float: left;” theme_builder_area=”post_content”][\/et_pb_image][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Stirred tank bioreactors are the industry classic<\/a>. They are generally cylindrical, contain at least one sparger to deliver gas into the culture medium, and utilize an impeller to mix the cell culture and break up\/distribute the gas bubbles throughout the bioreactor. These bioreactors may be either single‑use or stainless steel. Single‑use reactors range from about 15 mL to 2000 L, and reusable bioreactors may be as large as 50,000 L.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Advantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Stirred tank bioreactors are often used to scale a bioprocess from R&D to manufacturing scale. Because they are time tested and most commonly available, the math, physics, and biology of mass transfer is well known. These known mixing principles make for highly efficient gas transfer in stirred tank bioreactors. They are also highly flexible across a wide range of operating conditions, meaning they can be used to culture sensitive mammalian cells at low shear stresses, or to grow certain fungi requiring extreme temperatures and high pressures.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Disadvantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]While sparging allows for oxygen transfer through larger volumes and has a smaller footprint, it is not as efficient as oxygen transfer through the headspace. Another disadvantage is the shear stress introduced by the impellers, which must be minimized when stirring mammalian cultures. Impellers additionally introduce foam into bioreactors and must be treated with anti‑foaming agents. Finally, the impellers and spargers in stainless steel bioreactors must be thoroughly cleaned to prevent contamination.[\/et_pb_text][et_pb_heading title=”Rocker bioreactors” _builder_version=”4.27.4″ _module_preset=”default” title_level=”h3″ global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_image src=”https:\/\/www.alicat.com\/wp-content\/uploads\/2024\/05\/Types-of-Bio-Mixing-Rocking.webp” alt=”Rocking Bioreactor diagram” _builder_version=”4.27.4″ _module_preset=”default” max_width=”25%” max_width_tablet=”25%” max_width_phone=”50%” max_width_last_edited=”on|phone” module_alignment=”center” custom_margin=”8px||||false|false” custom_padding=”|2rem|2rem||false|false” custom_padding_tablet=”|2rem|2rem||false|false” custom_padding_phone=”|0rem|||false|false” custom_padding_last_edited=”on|phone” custom_css_main_element=”float: left;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”float: none;” custom_css_main_element_tablet=”float: left;” theme_builder_area=”post_content”][\/et_pb_image][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Rocker (or wave) bioreactors are also fairly common. They are generally large, plastic, single‑use bags that lay flat on a gently rocking platform (<100 rpm). These bioreactors deliver oxygen into the cell culture through the headspace and mix the broth using the rocker platform. Rocker bioreactors are most commonly used in laboratories. Since they are highly efficient but have a large footprint, they may also be used for small production volumes or to seed larger stirred tank bioreactors.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Advantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]The large, flat, 2‑dimensional rocker bioreactors have a very high surface‑area‑to‑volume ratio for highly efficient oxygen mass transfer. They are also gentler than stirred tank bioreactors because they fully aerate the culture without the need for impellers. Finally, as rocker bioreactors do not use spargers or impellers and are most often single use, they are simple to clean and pose almost no risk of cross‑contamination<\/a>.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Disadvantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Large, flat rocker bioreactors do not scale well. They generally range from only 5 to 100 L, with their size limited by the rate of oxygen transfer through the headspace.[\/et_pb_text][et_pb_heading title=”Shaker bioreactors” _builder_version=”4.27.4″ _module_preset=”default” title_level=”h3″ global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_image src=”https:\/\/www.alicat.com\/wp-content\/uploads\/2024\/05\/Types-of-Bio-Mixing-Spinning.webp” alt=”Spinning Bioreactor representation” _builder_version=”4.27.4″ _module_preset=”default” max_width=”25%” max_width_tablet=”25%” max_width_phone=”50%” max_width_last_edited=”on|phone” module_alignment=”center” custom_margin=”8px||||false|false” custom_padding=”|2rem|2rem||false|false” custom_padding_tablet=”|2rem|2rem||false|false” custom_padding_phone=”|0rem|||false|false” custom_padding_last_edited=”on|phone” custom_css_main_element=”float: left;” global_colors_info=”{}” custom_css_main_element_last_edited=”on|phone” custom_css_main_element_phone=”float: none;” custom_css_main_element_tablet=”float: left;” theme_builder_area=”post_content”][\/et_pb_image][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Shaker bioreactors sit on a platform that mixes by moving in a circular motion as simple as a 2D circle or as complex as a 3D figure eight. Like rocker bioreactors, they deliver gas entirely through the headspace.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Advantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]The swirling motion moves the cell culture broth up onto the sides of the bioreactor, resulting in a very thin fluid layer adhering to the side of the vessel. This enables maximum oxygen transfer without the need for a large footprint. Mass transfer is entirely dependent on gas flow through the headspace, so shaker bioreactors scale well linearly.<\/p>\n

This approach to mixing also mitigates shear stress, making it well suited for mammalian cell cultures.[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=”on|phone” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#F6F6F6″ width_tablet=”” width_phone=”” width_last_edited=”on|phone” custom_margin=”||2rem||false|false” custom_padding=”3rem|3rem|3rem|3rem|true|true” custom_padding_tablet=”3rem|3rem|3rem|3rem|true|true” custom_padding_phone=”2rem|2rem|2rem|2rem|true|true” custom_css_free_form=”selector .et_pb_text_inner {|| margin-bottom: 0;||}” border_radii=”on|15px|15px|15px|15px” border_width_all=”3px” border_color_all=”#2A3B47″ locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_heading title=”Shaker bioreactors, not shaker flasks” _builder_version=”4.27.4″ _module_preset=”default” title_level=”h3″ title_text_color=”#2A3B47″ global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_text_color=”#2A3B47″ text_font_size=”15px” global_colors_info=”{}” theme_builder_area=”post_content”]Shaker flasks are a low-cost, easy-to-use option for cell culture in a small-scale lab setting. They do not scale well, as they lack the full sensor package of a bioreactor and cannot monitor, control, and respond to environmental conditions. Shaker bioreactors are fully sensorized and scalable.[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”0px||0px||true|false” locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

Disadvantages<\/h4>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]While shaker bioreactors solve many of the issues associated with sparging and impelling, they are a much newer technology. The math of scale‑up and oxygen mass transfer are much less well‑known than for other bioreactor types.[\/et_pb_text][et_pb_heading title=”What bioreactor type should I use?” _builder_version=”4.27.4″ _module_preset=”default” title_level=”h3″ global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_heading][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||2rem||false|false” global_colors_info=”{}” theme_builder_area=”post_content”]Stirred tank bioreactors are proven to scale well, can be tightly controlled, and operate on known mass transfer principles. Rocker bioreactors have the most efficient mass transfer, but footprint rapidly becomes an issue during scale up. Shaker bioreactors are a newer technology, but one with the potential for high efficiency and yields, and linear scaling.<\/p>\n

Depending on the production volumes needed and the scale‑up plan, different types of bioreactors will likely be more or less advantageous during different development phases. Choosing bioreactors that are optimized for each development phase is a solid strategy. However, it may be viable to instead choose a bioreactor which is not well suited for a early phases but can easily scale from lab to manufacturing scale.[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”|700|||||||” custom_margin=”||2rem||false|false” locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”]Learn more: Mass flow considerations for spargers $<\/span><\/a>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

We\u2019ve previously written articles comparing single\u2011use bioreactors to stainless steel or glass multi\u2011use bioreactors. You can read about when to switch to single\u2011use, the advantages of single\u2011use in small\u2011scale bioprocessing, and the environmental impacts of single- and multi\u2011use bioreactors. In this article, we compare the following mixing technologies: shaker, rocker, and stirred tank bioreactors. At […]<\/p>\n","protected":false},"author":7,"featured_media":44428,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"1400","content-type":"","_searchwp_excluded":"","footnotes":""},"categories":[112,167],"tags":[],"class_list":["post-41720","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bioreactors-fermenters","category-bioreactors-fermenters-industry"],"acf":[],"yoast_head":"\nShakers, Rockers, and Stirred Tank Bioreactors \u2013 Advantages of Each - Alicat Scientific<\/title>\n<meta name=\"description\" content=\"Different types of bioreactors technologies, such as stirred tank bioreactors, shakers, and rockers, each have their own unique advantages.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.alicat.com\/de\/artikel\/schuttelwippe-ruhrkessel-bioreaktoren\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Shakers, Rockers, and Stirred Tank Bioreactors \u2013 Advantages of Each\" \/>\n<meta property=\"og:description\" content=\"Different types of bioreactors technologies, such as stirred tank bioreactors, shakers, and rockers, each have their own unique advantages.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.alicat.com\/de\/artikel\/schuttelwippe-ruhrkessel-bioreaktoren\/\" \/>\n<meta property=\"og:site_name\" content=\"Alicat Scientific\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/alicatscientific\/\" \/>\n<meta property=\"article:published_time\" content=\"2022-01-06T13:25:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-01-14T18:45:50+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.alicat.com\/wp-content\/uploads\/2022\/01\/article-typesofbioreactors.webp\" \/>\n\t<meta property=\"og:image:width\" content=\"950\" \/>\n\t<meta property=\"og:image:height\" content=\"839\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/webp\" \/>\n<meta name=\"author\" content=\"Alicat Scientific\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Alicat Scientific\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5\u00a0Minuten\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.alicat.com\\\/articles\\\/shaker-rocker-stirred-tank-bioreactors\\\/\",\"url\":\"https:\\\/\\\/www.alicat.com\\\/articles\\\/shaker-rocker-stirred-tank-bioreactors\\\/\",\"name\":\"Shakers, Rockers, and Stirred Tank Bioreactors \u2013 Advantages of Each - 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